DR. RAJENDER SINGH
 
....  
Sr. Scientist
Endocrinology Division
Central Drug Research Institute,
P.O. Box No. 173
Lucknow 226031
 
Educational Qualifications PhD ( CCMB, Hyderabad), M.Sc. ( GNDU, Amritsar),
Date of Birth 15-06-1981
Phone 091-522-2772450  Ext. 4393
Fax 091-522-2771941
E-Mail

rajender_singh@cdri.res.in

 
MY GROUP
Technical staff

Ms. Mohini Chhabra, M.Sc.

Ph.D. Students

Ms. Nishi Gupta, M.Sc. (Biotechnology)
Mr. Akhand Pratap Singh, M.Sc. (Biotechnology)
Mr. Muktanand Tripathi, M.Sc.

Project assistants

Mr. Saumya Sarkar, M.Sc.
Mr. Sandeep Kumar Bansal, M.Sc.
Mr. Ravinder Gulia, M.Sc.

 
AWARDS and Recognitions

Young Scientist Award from the Indian Society of Human Genetics (ISHG), India
Young Scientist Award from the Indian National Science Academy (INSA), India

 
CURRENT AREAS OF INTEREST

Identification of genetic causes of male infertility 

Y chromosome is a deletion hot spot and that contributes to approximately 15% of the cases of male infertility. Classical deletions have now been proven to result in male infertility. Partial deletions are relatively less studied particularly in Indian populations. We are studying the contribution of Y chromosome partial deletions towards male infertility. The study model involves identification of the clinical cases of male infertility who are subjected to genetic analysis of selected Y chromosome markers.

Non Y-chromosomal genetic causes of male infertility 

Y-chromosome based genetic causes have explained not more than 15-20% of the infertility cases. Recent research has identified expression of several autosomal genes in the reproductive organs and the number of such genes exceeds the number of Y-chromosomal genes. Identification of the autosomal genes that could contribute to male infertility is a major program in our laboratory. This includes genetic analysis on the infertile individuals with appropriate in vitro/in vivo functional assays to ascertain the pathogenic nature of selected genetic variants.

Treatment of male infertility

Male infertility may not be a problem in Indian perspective but it is very important issue in individual perspective. Moreover, male infertility cannot be looked just from treatment point of view; it may also provide important insights into the mechanism of spermatogenesis and genes required for male fertility. India is privileged with natural diversity and ancient literature such as Ayurveda which describes the wonder drugs identified via experiments by common man for thousands of years. We are conducting experiments to scientifically validate the claims of plant based drugs to treat different disorders of male reproduction such as male infertility and impotence.


SELECTED PUBLICATIONS
 
Nishi Gupta, Saraswati Gupta, Madhukar Dama, Archana David, Geeta Khanna, Anil Khanna, Singh Rajender. Strong association of 677 C>T substitution in the MTHFR gene with male infertility - a study on an Indian population and a meta-analysis. PLoS ONE, 2011(in press).
Rajender S, Monica MG, Walter L, Agarwal A. Thyroid, spermatogenesis, and male infertility. Front Biosci (Elite Ed).2011 Jun 1;3:843-55.
Rajender S, Avery K, Agarwal A. Epigenetics, spermatogenesis and male infertility. Mutat Res. 2011 May-Jun;727(3):62-71. Epub 2011 Apr 16.
Shukla KK, Mahdi AA, Mishra V, Rajender S, Sankhwar SN, Patel D, Das M. Withania somnifera improves semen quality by combating oxidative stress and cell death and improving essential metal concentrations. Reprod Biomed Online. 2011 Mar 7. [Epub ahead of print]
Dama MS, Singh NM, Rajender S. High fat diet prevents over-crowding induced decrease of sex ratio in mice. PLoS One. 2011 Jan 25;6(1):e16296.
Rajender S, Francis A, Pooja S, Krupakar N, Surekha D, Reddy G, Rao DR, Rao L, Ramachandra S, Vishnupriya S, Ramalingam K, Satyamoorthy K, Thangaraj K. CAG repeat length polymorphism in the androgen receptor gene and breast cancer risk: data on Indian women and survey from the world. Breast Cancer Res Treat. 2011 Jun;127(3):751-60. Epub 2010 Nov 24.
Sharma V, Singh R, Thangaraj K, Jyothy A. A novel Arg615Ser mutation of androgen receptor DNA-binding domain in three 46,XY sisters with complete androgen insensitivity syndrome and bilateral inguinal hernia. Fertil Steril. 2011 Feb;95(2):804.e19-21.
Pooja S, Francis A, Bid HK, Kumar S, Rajender S, Ramalingam K, Thangaraj K, Konwar R. Role of ethnic variations in TNF-α and TNF-β polymorphisms and risk of breast cancer in India. Breast Cancer Res Treat. 2011 Apr;126(3):739-47. Epub 2010 Sep 28.
Rajender S, Pooja S, Gupta NJ, Chakrabarty B, Singh L, Thangaraj K. G708E mutation in the androgen receptor results in complete loss of androgen function. J Androl. 2011 Mar-Apr;32(2):193-8. Epub 2010 Jul 29.
Chatterjee S, Singh R, Kadam S, Maitra A, Thangaraj K, Meherji P, Modi D. Longer CAG repeat length in the androgen receptor gene is associated with premature ovarian failure. Hum Reprod. 2009 Aug 14. [Epub ahead of print]
Rajender S, Vijayalakshmi K, Pooja S, Madhavi S, Paul SF, Vettriselvi V, Shroff S, Singh L, Thangaraj K. Longer (TA)n Repeat but not A49T and V89L Polymorphisms in SRD5A2 Gene May Confer Prostate Cancer Risk in South Indian Men. J Androl. 2009 May 14. [Epub ahead of print]
Ahmad MK, Mahdi AA, Shukla KK, Islam N, Rajender S, Madhukar D, Shankhwar SN, Ahmad S. Withania somnifera improves semen quality by regulating reproductive hormone levels and oxidative stress in seminal plasma of infertile males. Fertil Steril. 2009 Jun 5. [Epub ahead of print
Rajender S, Gupta NJ, Chakrabarty B, Singh L, Thangaraj K. Ala 586 Asp mutation in androgen receptor disrupts transactivation function without affecting androgen binding. Fertil Steril. 2009 Mar;91(3):933.e23-8. Epub 2008 Dec 4.
Madhukar D, Rajender S. Hormonal treatment of male infertility: promises and pitfalls. J Androl. 2009 Mar-Apr;30(2):95-112. Epub 2008 Oct 16. Review
Rajender S, Singh L, Thangaraj K. L859F Mutation in Androgen Receptor Gene Results in Complete Loss of Androgen binding to the Receptor. J Androl. 2007; 28: 772-776.
Rajender S, Singh L, Thangaraj K. Phenotypic heterogeneity of androgen receptor gene mutations. Asian J Androl. 2007; 9:147-79. Review.
Rajender S, Thangaraj K, Gupta NJ, Leelavathy N, Deepa SR, Nambiar RG, Kalavathy V, Santhiya ST, Rajangam S, Gopinath PM, Chakravarty B, Singh L. A novel sex-determining gene mapped to Xp11.21-Xp11.23. Journal of Clinical Endocrinology and Metabolism 2006; 91: 4028-36.
Rajender S, Rajani V, Gupta NJ, Chakravarty B, Singh L, Thangaraj K. SRY-negative 46,XX male with normal genitals, complete masculinization and infertility. Mol Hum Reprod. 2006; 12: 341-6.
Rajender S, Shastry PK, Rasalkar AA, Singh L, Thangaraj K. A novel androgen receptor mutation resulting in complete androgen insensitivity syndrome and bilateral Leydig cell hyperplasia. J Androl. 2006; 27: 510-516
Rajender S, Rajani V, Gupta NJ, Chakravarty B, Singh L, Thangaraj K. No association of androgen receptor GGN repeat length polymorphism with infertility in Indian men. J Androl. 2006 Nov-Dec;27(6):785-9. Epub 2006 Jun 28.
Singh R, Deepa SR, Madhavi S, Gupta NJ, Chakravarty B, Singh L, Thangaraj K. Male infertility: no evidence of involvement of androgen receptor gene among Indian men. J Androl. 2006 Jan-Feb;27(1):102-5.
Vijayalakshmi K, Thangaraj K, Rajender S, Vettriselvi V, Venkatesan P, Shroff S, Vishwanathan KN, Paul SF. GGN repeat length and GGN/CAG haplotype variations in the androgen receptor gene and prostate cancer risk in south Indian men. J Hum Genet. 2006;51(11):998-1005. Epub 2006 Sep 13.
Mittal RD, Mishra DK, Thangaraj K, Singh R, Mandhani A. Is there an inter-relationship between prostate specific antigen, kallikrein-2 and androgen receptor gene polymorphisms with risk of prostate cancer in north Indian population? Steroids. 2007 Apr;72(4):335-41. Epub 2006 Dec 19.