Model |
Assay System |
Disease |
Quantity
Required |
Scientist(s)
involved |
FRET assays for screening of small anti-cancer molecules |
Assay |
Anti -cancer |
20 mg |
Dr. G. Gupta |
MTT assay |
Assay |
Anti-cancer |
5mg |
Dr S Sanyal |
Sulphorhodamine B assay (SRB) |
Assay |
Anti-cancer |
5mg |
Dr S Sanyal |
Trypan Blue exclusion |
Assay |
Anti-cancer |
5mg |
Dr S Sanyal |
BRDU incorporation assay |
Assay |
Anti-cancer |
5mg |
Dr S Sanyal |
Anexin V/PI assay |
Assay |
Anti-cancer |
5mg |
Dr S Sanyal |
Real-time PCR-based methods for specific apoptotic/survival gene expressions |
Assay |
Anti-cancer |
5mg |
Dr S Sanyal |
| Western based methods for specific apoptotic/survival gene expressions |
Assay |
Anti-cancer |
5mg |
Dr S Sanyal |
Trypan Blue exclusion . |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
Anexin V/PI assay |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
pTK-(C/EBP)-Luc assay |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
Real-time PCR-based methods for specific apoptotic/survival gene expressions |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
Western based methods for specific apoptotic/survival gene expressions |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
Cytospin based Giemsa staining for assessment of differentiation |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
FACS based assay for analysis of differentiation and Apoptosis |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
Gelshift Assay |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
In-vivo ubiquitination Assay |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
Crystal violet Assay for assessment of cell growth |
Assay |
Anti Cancer |
|
Dr Arun Trivedi |
Patient derived primary cultures for drug screening |
Assay |
Anti -cancer |
This will depend on the in vitro spermicidal conc. |
Dr. G. Gupta |
In vitro cell based assays for screening of nuclear receptors |
Assay |
Anti -cancer |
50 mg (pure compounds) |
Dr. S.Sanyal |
standardized.Proteomic screening of anticancer agents |
Assay |
Anti -cancer |
1.00 gram |
Dr. G. Gupta |
Wound healing assay |
In vitro |
Cancer |
20 mg |
Drs. S.Musthapa, R. Konwar, D.P. Mishra. |
Migration and invasion assays |
In vitro |
Cancer |
20 mg |
Dr s. S.Musthapa, D.P. Mishra |
DMBA-induced mammary tumor model |
In vivo |
Breast cancer |
500 mg |
Dr. R. Konwar |
Topoisomerase inhibition assay |
In vitro |
Cancer |
20 mg |
Dr. R. Konwar |
Anti-proliferation Assay in Primary cell lines |
In vitro screening for the compounds anti-proliferation activity |
Cancer |
20 mg |
Drs. S.Musthapa, R. Konwar, D.P. Mishra |
Apoptosis assay in primary cells/ tumors
-To evaluate the anti-cancerous properties of a particular compound using apoptosis assay |
In vitro/ In vivo |
Cancer |
30 mg/ 500 mg |
Drs. S.Musthapa, D.P. Mishra |
DNMT, HDAC, HAT activity assays
- Epigenetic assays for aging and anti-cancer research.
Assays being used to evaluate the epigenetic targets of novel compounds in mammalian cell lines. The major epigenetic targets are DNMTs, HATs and HDACs. |
In vitro |
Cancer, Aging |
30 mg |
Dr. S. Musthapa |
Telomerase assay |
In vitro |
Cancer, Aging |
30 mg |
Dr. S. Musthapa |
Chromatin Immuno Precipitation (ChIP) assay- For DNA-protein interaction studies |
In vitro |
Cancer / Male Reproduction/Neurobiology |
30 mg |
Dr. D. P. Mishra |
Comet Assay
-For DNA damage |
In vitro |
Cancer, Male reproduction |
20 mg |
Drs. D.P. Mishra, S.Musthapa |
Transcription assay |
In vitro |
Cancer, Male reproduction |
20 mg |
Dr. D. P. Mishra |
Clonogenic assay |
In vitro |
Cancer |
20 mg |
Drs. S. Musthapa,
D.P. Mishra |
Proteasome inhibition assay –For screening of proteasome inhibitors |
In vitro |
Cancer / Male Reproduction |
20 mg |
Dr. D. P. Mishra |
Mammalian two hybrid assay- Cellular function |
In vitro |
Cancer / Male Reproduction |
20 mg |
Dr. D. P. Mishra |
Flowcytometric TUNEL assay-DNA damage, apoptosis |
In vitro |
Cancer / Male Reproduction |
20 mg |
Drs. S. Musthapa,
D.P. Mishra |
PMN Phagocytosis assay- For screening of Phagocytosis |
In vitro |
Cancer / Male Reproduction |
20 mg |
Dr. D. P. Mishra |
Flurometry based autophagy assay- For screening of Autophagy |
In vitro |
Cancer / Male Reproduction |
20 mg |
Dr. D. P. Mishra |
| Luciferase reporter assay- For evaluation of Transcriptional activity |
In vitro |
Cancer / Male Reproduction/Neurobiology |
20 mg |
Dr. D. P. Mishra |
BACK
TO BIOLOGICAL ASSAYS |
