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BIOLOGICAL ASSAYS AVAILABLE
 
REPRODUCTIVE HEALTH  

Researchers are invited to make use of the assays detailed below. In case of any technical query, the scientists concerned may be approached through email.

 
   
Model/Assay
Assay System
Disease area Quantity
Required
Scientist(s)
involved
Sander-Cramer assay for evaluation of spermicidal activity In vitro Contraception 20 mg Dr. G. Gupta
Assay for vaginal contraception in Rabbit In vivo Contraception This will depend on the in vitro spermicidal conc. Dr. G. Gupta
Evaluation of Anti-proliferative activity against human BPH-stromal primary cells In vitro Benign Prostatic Hyperplasia 50 mg (pure compounds) Dr. G. Gupta
In vivo assay for anti-BPH activity in rat model In vivo Benign Prostatic Hyperplasia 1.00 gram Dr. G. Gupta
Anti-spermatogenic activity in rats In vivo Contraception 3.0 grams Dr. G. Gupta
Anti-implantation screening assays In vivo Contraception 3.0 grams Drs. A. Dwivedi,  M.Sachdev
Wound healing assay In vitro Cancer 20 mg Drs. S.MusthapaR. Konwar,    
D.P. Mishra.
Migration and invasion assays In vitro Cancer 20 mg Dr s. S.Musthapa, D.P. Mishra
DMBA-induced mammary tumor model In vivo Breast cancer 500 mg Dr. R. Konwar
Topoisomerase inhibition assay In vitro Cancer 20 mg Dr. R. Konwar
Anti-proliferation Assay in Primary cell lines In vitro screening for the compounds anti-proliferation activity Cancer 20 mg Drs. S.Musthapa, R. Konwar,
D.P. Mishra
Apoptosis assay in primary cells/ tumors
-To evaluate the anti-cancerous properties of a particular compound using apoptosis assay
In vitro/ In vivo Cancer 30 mg/ 500 mg Drs. S.Musthapa,  D.P. Mishra
DNMT, HDAC, HAT activity assays
- Epigenetic assays for aging and anti-cancer research.
Assays being used to evaluate the epigenetic targets of novel compounds in mammalian cell lines. The major epigenetic targets are DNMTs, HATs and HDACs.
In vitro Cancer, Aging 30 mg Dr. S. Musthapa
Telomerase assay In vitro Cancer, Aging 30 mg Dr. S. Musthapa
Chromatin Immuno Precipitation (ChIP) assay- For DNA-protein interaction studies In vitro Cancer / Male Reproduction/Neurobiology 30 mg Dr. D. P. Mishra
Comet Assay
-For DNA damage
In vitro Cancer, Male reproduction 20 mg Drs. D.P. Mishra, S.Musthapa
Transcription assay In vitro Cancer, Male reproduction 20 mg Dr. D. P. Mishra
Clonogenic assay In vitro Cancer 20 mg Drs. S. Musthapa,
D.P. Mishra
Proteasome inhibition assay –For screening of proteasome inhibitors In vitro Cancer / Male Reproduction 20 mg Dr. D. P. Mishra
Mammalian two hybrid assay- Cellular function In vitro Cancer / Male Reproduction 20 mg Dr. D. P. Mishra
Flowcytometric TUNEL assay-DNA damage, apoptosis In vitro Cancer / Male Reproduction 20 mg Drs. S. Musthapa,
D.P. Mishra
PMN Phagocytosis assay-   For screening of Phagocytosis In vitro Cancer / Male Reproduction 20 mg Dr. D. P. Mishra
Flurometry based autophagy assay- For screening of  Autophagy  In vitro Cancer / Male Reproduction 20 mg Dr. D. P. Mishra
Middle cerebral artery occlusion- For Screening of neuroprotective activity  In vitro Neurobiology 20 mg Dr. D. P. Mishra
Luciferase reporter assay- For evaluation of Transcriptional activity In vitro Cancer / Male Reproduction/Neurobiology 20 mg Dr. D. P. Mishra
Neonatal SD rats for calvarial osteoblast cell isolation.
-Use for assessing alkaline phosphatase activity as a measure of osteoblast differentiation and alizarin staining for osteoblast mineralization
In vitro Osteoporosis/ Bone diseases 10 mg Drs. N. Chattopadhyay,
D. Singh,
R. Trivedi
Peak bone mass measurements- Rats administered with compound showing promising in vitro activity. Treatment for one month, autopsied and bones and serum used for evaluating bone formation and resorption parameters. In vivo Osteoporosis/ Bone diseases 1 gram Drs. N. Chattopadhyay,
D. Singh,
R. Trivedi
Adult SD osteopenic rat model. -Rats ovariectomized and left for a period of three months to develop osteopenia. Following this, osteopenic rats administered compound for three months, autopsied and bones and serum used for evaluating bone formation and resorption parameters In vivo Osteoporosis/ Bone diseases 5 g Drs. N. Chattopadhyay,
D. Singh,
R. Trivedi
Adult Ovariectomized Balb/c mice model. Mice ovariectomized and administered compound for one month, autopsied and bones and serum used for evaluating bone formation and resorption parameters In vivo Osteoporosis/ Bone diseases 100 mg Drs. N. Chattopadhyay,
D. Singh,
R. Trivedi
Bone marrow culture from adult SD rats-For osteogenic differentiation Ex vivo Osteoporosis/ Bone diseases 10 mg Drs. N. Chattopadhyay,
D. Singh,
R. Trivedi
Bone marrow culture from female Balb/c mice-For osteoclast differentiation Ex vivo Osteoporosis/ Bone diseases 10 mg Drs. N. Chattopadhyay,
D. Singh,
R. Trivedi
Relative binding affinity for estrogen receptor In vitro Female infertility/ Estrogen- related disorders 10 mg Dr. A. Dwivedi
Estrogenic/ antiestrogenic activity in rat model In vivo Female infertility/ Estrogen- related disorders 500 mg Dr. A. Dwivedi
Cell based estrogen receptor transactivation assay-To evaluate estrogenic/ antiestrogenic activity In vitro Female infertility/ Estrogen- related disorders 20 mg Dr. A. Dwivedi