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| LABORATORY
ANIMAL SERVICES |
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| Laboratory
Animals Facilities |
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The NLAC possesses complete
facilities for breeding and maintenance
of laboratory animals under standard husbandry
conditions. The centre houses animal strains
of different species of rodents, including
inbred strains of mice, rat, hamster, logomorphs
and nude mice. The centre also possesses
specific pathogen free (SPF) and complete
germfree (gnotobiotic) rodent breeding nuclei
and a facility for primate behaviour studies
and primate breeding.
This centre is self contained
with its own Radiology, Genetics, Microbiology,
Pathology, Nutrition, Parasitology, Services
etc. for disease monitoring and management
of laboratory animals. The environment provided
to animals housed in the centre - including
cages, feed, water etc. - matches with specifications
laid down by Bureau of Indian Standards
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This centre takes care
of humane handling of animals and follows
Indian National science Academy guidelines
for this purpose. NLAC is registered with
the Committee for the Purpose of Control
and Supervision of
Experiments on Animals (CPCSEA)
for breeding and experiments on animals.
NLAC supplies
animals for research projects of CDRI and
to the outside institutions throughout India
and abroad after necessary regulatory clearance.
The centre also imparts training to nationals
as well as International scientists/technicians
and provide consultancy in this area.
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| Tissue
Culture Unit
also has established a Tissue Culture Unit
where human and animal cell lines are maintained.
These cell lines are made available to the
scientists of CDRI as well as the outside
research institutes. The major purpose of
this unit is to provide alternatives to
the animal usage to scientists. The use
of animals is minimised wherever possible
by using cell lines in place of animals.
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unit caters to the following functions
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It serves as a repository
for transformed cell lines inlcuding maintenance,
cryostorage and supply of cells for in vitro
studies.
The repository consists of the following cell
lines: MCF-7 (Human Breast Adenocarcinoma),
Vero C-1008 (NCCS and NIV strains), L-929
mouse fibroblasts, 3T3-mouse embryo fibroblasts,
etc.. New cell lines can be added and made
available on demand. |
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Development of customized
primary culture procedures for hepatocytes,
splenocytes, mast cells from rat, mouse, etc.
Development of serum-free explant culture
system from murine liver/spleen explants,
rabbit endometrial explants, etc. |
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| Supplying cell lines
and procedures for cell culture, etc. to laboratories/institutions |
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Short and Long term
training facilities for trainees in tissue
culture.
Trainees include undergraduate/postgraduate
students, candidates sponsored from industry
and foreign trainees |
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| Facilities
Available in the unit |
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The existing facilities cover complete
infrastructure for performing tissue and
cell culture experiments:
Laminar flow in appropriate culture room
A walk-in cold room at 4°c.
CO2 Incubator and inverted phase contrast
microscope
Liquid Nitrogen storage facilities for
storage of culture samples, etc.
A full-fledged Laboratory for conducting
biochemical studies on tissue and cells.
Equipments such as phase contrast microscope
for cytological studies, sonicator, PAGE
and Western Blotting equipment, etc.
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website
copyright © Central Drug Research Institute.
All rights reserved.
No part of this should be Downloaded or used
in any way other than the non-commercial purposes
without prior permission of the Director,
CDRI. |
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