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| DR.
VINOD BHAKUNI |
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Scientist F
Scientist-in-Charge
Molecular and Structural Biology Division
P.O. Box No. 173
Chhattar Manzil
Lucknow -226001
India |
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| POST-DOCTORAL
RESEARCH |
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John Hopkins
University, Baltimore, USA 1990 - 1992 |
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| RESEARCH
AREAS |
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Protein Folding/
Engineering ; Biophysical Chemistry |
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| CURRENT
AREAS OF INTEREST |
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Mechanism
of Subunit Assembly of multimemeric Proteins |
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Role of Domain
Structures in Protein Stability and Function |
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| SELECTED
PUBLICATIONS |
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Bhatt AN and Bhakuni
V. Characterization of Pyridoxal 5'-Phosphate-Binding
Domain and Folding Intermediate of Bacillus subtilis Serine
Hydroxymethyltransferase: an Autonomous Folding Domain
(2008) J Biochem. 144,
295-303 |
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Kumar R and Bhakuni
V. Mycobacterium tuberculosis isocitrate lyase
(MtbIcl): role of divalent cations in modulation of functional
and structural properties Proteins: Struct. Funct. (2008)
Bioinformatics 72, 892-900 |
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Singh K and Bhakuni
V. Toxoplasma gondii Ferredoxin-NADP+ Reductase
(TgFNR): Role of ionic interactions in stabilization of
native conformation and structural cooperativity. Proteins:
Struct. Funct. (2008) Bioinformatics
71, 1879-88 |
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Tripathi T, Rahlfs S,
Becker K and Bhakuni V. Structural and
stability characteristics of a monothiol glutaredoxin:
glutaredoxin-like protein 1 from Plasmodium falciparum.
(2008) Biochimica et Biophysica
Acta (BBA) - Proteins & Proteomics 1784,
946-52. |
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Akhtar MS and
Bhakuni V - Role of ionic interactions and linker
in the domain interaction and modulation of functional
activity of hyaluronate lyases . (2007)
- Biochemical and Biophysical Research Communications
353, 286-292 |
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Sharma Sarita and Bhakuni
Vinod - Cloning and structural analysis of Mycobacterium
leprae serine hydroxymethyltransferase. (2007)
- Protein Expression and Purification
55, 189-197 |
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Singh K and Bhakuni
V - Cation induced differential effect on structural
and functional properties of Mycobacterium tuberculosis
a-isopropylmalate synthase. (2007) - BMC Struct
Biol 19, 39 |
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Tripathi T, Rahlfs S,
Becker K and Bhakuni V - Glutathione
mediated regulation of oligomeric structure and functional
activity of Plasmodium falciparum glutathione S-transferase.
(2007) - BMC Struct Biol
17, 67 |
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Mishra, Parul; Akhtar,
Md Sohail; Bhakuni, Vinod (2006),
Unusual structural features of the bacteriophage-associated
hyaluronate lyase (hylp2), Journal Of Biological
Chemistry , Volume 281, Issue 11,7143-7150 |
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Akhtar MS,
Krishnan MY, Bhakuni V.(2006) Insight
into the mechanism of action of hyaluronate lyase: Role
of C-terminal domain and Ca++ in the functional regulation
of enzyme. Journal Of Biological Chemistry, 2006
Jul 19; [Epub ahead of print] |
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Bhatt Anant Narayan, Shukla
Nidhi, Aliverti Alessandro, Zanetti Giuliana,
Bhakuni Vinod. (2005) Modulation of cooperativity
in Mycobacterium tuberculosis NADPH-ferredoxin reductase:
Cation-and pH-induced alterations in native conformation
and destabilization of the NADP+-binding domain.
Protein Science, 14, 980-92. |
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Shukla Nidhi, Bhatt Anant
Narayan, Aliverti Alessandro, Zanetti Giuliana & Bhakuni
Vinod. (2005) Guanidinium chloride-
and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin
reductase. FEBS J, 272, 2216-24.
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Avasthi, A, Dutta, G P,
Bhakuni, V, Tripathi,
R ( 2004), Resistance reversal action
of ketoconazole againstmefloquine resistance of Plasmodium
yeolii nigerensis, Parasitology, 107, 115-19 |
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Bhatt, A.N., Khan, M.Y.,
and Bhakuni, V. (2004) The Terminal Lysines
of Dimeric Serine Hydroxymethyl Transferase Play an Important
Role in Stabilization of the Quarternary Structure : Differences
in Alkaline Denaturation of Mesophilic and Thermophilic
Proteins and its Chimeras. Protein Science ,13 (8),
2184-95 |
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Chaturvedi,
S. & Bhakuni, V. (2003) Unusual Structural,
Functional and Stability Properties of Serine Hydroxymethyltransferase
from Mycobacterium tuberculosis. J. Biol. Chem.
278 (42), 40793-40805 |
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Akhtar, Md.
S. & Bhakuni, V. (2003) Streptococcus
pneumoniae hyaluronate lyase contains two non-cooperative
independent folding/unfolding domains. Characterization
of functional domain and inhibitors of enzyme. J. Biol.
Chem., 278, 25509-25516 |
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Misra, S.K.
& Vinod Bhakuni (2003) Unique Holoenzyme
Dimers of Tetrameric Enzyme E. coli Methylenetetrahydrofolate
reductase: Characterization of structural features associated
with modulation of the enzyme s function. Biochemistry
42, 3921-3928. |
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Bhatt, A.N.,
Prakash, K., Subramanya, H.S. & Vinod Bhakuni
(2002) Different unfolding pathways for mesophilic
and thermophilic homologs of serine hydroxymethyltransferase
Biochemistry 41,
12115-12123. |
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Akhtar, Md.
S., Ahmad A. & Vinod Bhakuni. (2002)
Divalent cations induced changes in structural properties
of dimeric enzyme glucose oxidase: Dual effect of stabilization
and dissociation and loss of cooperative interactions
in enzyme monomer Biochemistry 41, 7142-7149. |
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Akhtar, Md.
S., Ahmad A. & Vinod Bhakuni. (2002)
Guanidine hydrochloride- and Urea-induced unfolding of
dimeric enzyme Glucose Oxidase. Biochemistry 41,
3819-3827. |
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Prakash,
K, Prajapati, S, Ahmad, A, Jain, S.K & Vinod Bhakuni
(2002) Unique oligomeric intermediates of bovine
liver catalase. Protein Science 11, 46-57. |
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Ahmad, A.,
Akhtar, Md. S. & Vinod Bhakuni (2001)
Monovalent cation-induced conformational changes in glucose
oxidase leading to stabilization of the enzyme. Biochemistry
40, 1945-1955. |
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Ali, V.,
Prakash, K., Kulkarni, S., Ahmad , A., Madhusudan, K.P.
& Vinod Bhakuni (1999) 8-anilino-1-naphthalene
sulphonic acid (ANS) induces folding of acid unfolded
cytochrome c to molten globule state as a result of electrostatic
interactions. Biochemistry 38, 13635-13642. |
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