DR. VINOD BHAKUNI
 
Scientist F
Scientist-in-Charge
Molecular and Structural Biology Division
P.O. Box No. 173
Chhattar Manzil
Lucknow -226001
India
 
E Mail vinod_bhakuni@cdri.res.in
Educational Qualifications M. Sc. Ph.D
Date of Birth 24.05.1962
 
POST-DOCTORAL RESEARCH
 
John Hopkins University, Baltimore, USA 1990 - 1992
RESEARCH AREAS
 
Protein Folding/ Engineering ; Biophysical Chemistry
 
CURRENT AREAS OF INTEREST
 
Mechanism of Subunit Assembly of multimemeric Proteins
Role of Domain Structures in Protein Stability and Function
 
SELECTED PUBLICATIONS
 
Bhatt AN and Bhakuni V. Characterization of Pyridoxal 5'-Phosphate-Binding Domain and Folding Intermediate of Bacillus subtilis Serine Hydroxymethyltransferase: an Autonomous Folding Domain (2008) J Biochem. 144, 295-303
Kumar R and Bhakuni V. Mycobacterium tuberculosis isocitrate lyase (MtbIcl): role of divalent cations in modulation of functional and structural properties Proteins: Struct. Funct. (2008) Bioinformatics 72, 892-900
Singh K and Bhakuni V. Toxoplasma gondii Ferredoxin-NADP+ Reductase (TgFNR): Role of ionic interactions in stabilization of native conformation and structural cooperativity. Proteins: Struct. Funct. (2008) Bioinformatics 71, 1879-88
Tripathi T, Rahlfs S, Becker K and Bhakuni V. Structural and stability characteristics of a monothiol glutaredoxin: glutaredoxin-like protein 1 from Plasmodium falciparum. (2008) Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics 1784, 946-52.
Akhtar MS and Bhakuni V - Role of ionic interactions and linker in the domain interaction and modulation of functional activity of hyaluronate lyases . (2007) - Biochemical and Biophysical Research Communications 353, 286-292
Sharma Sarita and Bhakuni Vinod - Cloning and structural analysis of Mycobacterium leprae serine hydroxymethyltransferase. (2007) - Protein Expression and Purification 55, 189-197
Singh K and Bhakuni V - Cation induced differential effect on structural and functional properties of Mycobacterium tuberculosis a-isopropylmalate synthase. (2007) - BMC Struct Biol 19, 39
Tripathi T, Rahlfs S, Becker K and Bhakuni V - Glutathione mediated regulation of oligomeric structure and functional activity of Plasmodium falciparum glutathione S-transferase. (2007) - BMC Struct Biol 17, 67
Mishra, Parul; Akhtar, Md Sohail; Bhakuni, Vinod (2006), Unusual structural features of the bacteriophage-associated hyaluronate lyase (hylp2), Journal Of Biological Chemistry , Volume 281, Issue 11,7143-7150
Akhtar MS, Krishnan MY, Bhakuni V.(2006) Insight into the mechanism of action of hyaluronate lyase: Role of C-terminal domain and Ca++ in the functional regulation of enzyme. Journal Of Biological Chemistry, 2006 Jul 19; [Epub ahead of print]
Bhatt Anant Narayan, Shukla Nidhi, Aliverti Alessandro, Zanetti Giuliana, Bhakuni Vinod. (2005) Modulation of cooperativity in Mycobacterium tuberculosis NADPH-ferredoxin reductase: Cation-and pH-induced alterations in native conformation and destabilization of the NADP+-binding domain.
Protein Science, 14, 980-92.
Shukla Nidhi, Bhatt Anant Narayan, Aliverti Alessandro, Zanetti Giuliana & Bhakuni Vinod. (2005) Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase. FEBS J, 272, 2216-24.
Avasthi, A, Dutta, G P, Bhakuni, V, Tripathi, R ( 2004), Resistance reversal action of ketoconazole againstmefloquine resistance of Plasmodium yeolii nigerensis, Parasitology, 107, 115-19
Bhatt, A.N., Khan, M.Y., and Bhakuni, V. (2004) The Terminal Lysines of Dimeric Serine Hydroxymethyl Transferase Play an Important Role in Stabilization of the Quarternary Structure : Differences in Alkaline Denaturation of Mesophilic and Thermophilic Proteins and its Chimeras. Protein Science ,13 (8), 2184-95
Chaturvedi, S. & Bhakuni, V. (2003) Unusual Structural, Functional and Stability Properties of Serine Hydroxymethyltransferase from Mycobacterium tuberculosis. J. Biol. Chem. 278 (42), 40793-40805
Akhtar, Md. S. & Bhakuni, V. (2003) Streptococcus pneumoniae hyaluronate lyase contains two non-cooperative independent folding/unfolding domains. Characterization of functional domain and inhibitors of enzyme. J. Biol. Chem., 278, 25509-25516
Misra, S.K. & Vinod Bhakuni (2003) Unique Holoenzyme Dimers of Tetrameric Enzyme E. coli Methylenetetrahydrofolate reductase: Characterization of structural features associated with modulation of the enzyme s function. Biochemistry 42, 3921-3928.
Bhatt, A.N., Prakash, K., Subramanya, H.S. & Vinod Bhakuni (2002) Different unfolding pathways for mesophilic and thermophilic homologs of serine hydroxymethyltransferase Biochemistry 41,
12115-12123.
Akhtar, Md. S., Ahmad A. & Vinod Bhakuni. (2002) Divalent cations induced changes in structural properties of dimeric enzyme glucose oxidase: Dual effect of stabilization and dissociation and loss of cooperative interactions in enzyme monomer Biochemistry 41, 7142-7149.
Akhtar, Md. S., Ahmad A. & Vinod Bhakuni. (2002) Guanidine hydrochloride- and Urea-induced unfolding of dimeric enzyme Glucose Oxidase. Biochemistry 41, 3819-3827.
Prakash, K, Prajapati, S, Ahmad, A, Jain, S.K & Vinod Bhakuni (2002) Unique oligomeric intermediates of bovine liver catalase. Protein Science 11, 46-57.
Ahmad, A., Akhtar, Md. S. & Vinod Bhakuni (2001) Monovalent cation-induced conformational changes in glucose oxidase leading to stabilization of the enzyme. Biochemistry 40, 1945-1955.
Ali, V., Prakash, K., Kulkarni, S., Ahmad , A., Madhusudan, K.P. & Vinod Bhakuni (1999) 8-anilino-1-naphthalene sulphonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions. Biochemistry 38, 13635-13642.