DR. VINOD BHAKUNI
 
Scientist-in-Charge
Molecular and Structural Biology Division
P.O. Box No. 173
Chhattar Manzil
Lucknow -226001
India
 
E Mail bhakuniv[at]rediffmail dot com
Educational Qualifications M. Sc. Ph.D
 
POST-DOCTORAL RESEARCH
 
John Hopkins University, Baltimore, USA 1990 - 1992
RESEARCH AREAS
 
Protein Folding/ Engineering ; Biophysical Chemistry
 
CURRENT AREAS OF INTEREST
 
Mechanism of Subunit Assembly of multimemeric Proteins
Role of Domain Structures in Protein Stability and Function
 
SELECTED PUBLICATIONS
 
Mishra, Parul; Akhtar, Md Sohail; Bhakuni, Vinod (2006), Unusual structural features of the bacteriophage-associated hyaluronate lyase (hylp2), Journal Of Biological Chemistry , Volume 281, Issue 11,7143-7150
Akhtar MS, Krishnan MY, Bhakuni V.(2006) Insight into the mechanism of action of hyaluronate lyase: Role of C-terminal domain and Ca++ in the functional regulation of enzyme. Journal Of Biological Chemistry, 2006 Jul 19; [Epub ahead of print]
Bhatt Anant Narayan, Shukla Nidhi, Aliverti Alessandro, Zanetti Giuliana, Bhakuni Vinod. (2005) Modulation of cooperativity in Mycobacterium tuberculosis NADPH-ferredoxin reductase: Cation-and pH-induced alterations in native conformation and destabilization of the NADP+-binding domain.
Protein Science, 14, 980-92.
Shukla Nidhi, Bhatt Anant Narayan, Aliverti Alessandro, Zanetti Giuliana & Bhakuni Vinod. (2005) Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase. FEBS J, 272, 2216-24.
Avasthi, A, Dutta, G P, Bhakuni, V, Tripathi, R ( 2004), Resistance reversal action of ketoconazole againstmefloquine resistance of Plasmodium yeolii nigerensis, Parasitology, 107, 115-19
Bhatt, A.N., Khan, M.Y., and Bhakuni, V. (2004) The Terminal Lysines of Dimeric Serine Hydroxymethyl Transferase Play an Important Role in Stabilization of the Quarternary Structure : Differences in Alkaline Denaturation of Mesophilic and Thermophilic Proteins and its Chimeras. Protein Science ,13 (8), 2184-95
Chaturvedi, S. & Bhakuni, V. (2003) Unusual Structural, Functional and Stability Properties of Serine Hydroxymethyltransferase from Mycobacterium tuberculosis. J. Biol. Chem. 278 (42), 40793-40805
Akhtar, Md. S. & Bhakuni, V. (2003) Streptococcus pneumoniae hyaluronate lyase contains two non-cooperative independent folding/unfolding domains. Characterization of functional domain and inhibitors of enzyme. J. Biol. Chem., 278, 25509-25516
Misra, S.K. & Vinod Bhakuni (2003) Unique Holoenzyme Dimers of Tetrameric Enzyme E. coli Methylenetetrahydrofolate reductase: Characterization of structural features associated with modulation of the enzyme s function. Biochemistry 42, 3921-3928.
Bhatt, A.N., Prakash, K., Subramanya, H.S. & Vinod Bhakuni (2002) Different unfolding pathways for mesophilic and thermophilic homologs of serine hydroxymethyltransferase Biochemistry 41,
12115-12123.
Akhtar, Md. S., Ahmad A. & Vinod Bhakuni. (2002) Divalent cations induced changes in structural properties of dimeric enzyme glucose oxidase: Dual effect of stabilization and dissociation and loss of cooperative interactions in enzyme monomer Biochemistry 41, 7142-7149.
Akhtar, Md. S., Ahmad A. & Vinod Bhakuni. (2002) Guanidine hydrochloride- and Urea-induced unfolding of dimeric enzyme Glucose Oxidase. Biochemistry 41, 3819-3827.
Prakash, K, Prajapati, S, Ahmad, A, Jain, S.K & Vinod Bhakuni (2002) Unique oligomeric intermediates of bovine liver catalase. Protein Science 11, 46-57.
Ahmad, A., Akhtar, Md. S. & Vinod Bhakuni (2001) Monovalent cation-induced conformational changes in glucose oxidase leading to stabilization of the enzyme. Biochemistry 40, 1945-1955.
Ali, V., Prakash, K., Kulkarni, S., Ahmad , A., Madhusudan, K.P. & Vinod Bhakuni (1999) 8-anilino-1-naphthalene sulphonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions. Biochemistry 38, 13635-13642.