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| Dr. Monika Sachdev |
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Senior Scientist,
Division of Endocrinology,
Central Drug Research Institute,
Lucknow - 226 031, India |
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| Educational Qualifications |
- Post-doctoral study as a NIH-Fogarty Fellow in the field of Reproductive Biology & Early Embryology from Univ. of Virginia, USA during the years 2004-2009
- Ph.D. in Animal Sciences from Hyderabad Central University, Hyderabad, in 2004
- M.Sc. in Molecular Biology & Biotechnology from G.B. Pant Univ. of Agri. & Tech., Pantnagar, in 1997
- B.Sc. in Botany, Zoology and Chemistry from Kanpur University, Kanpur, during the years 1991-1994
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91-9026561774
E-mail: |
| E-Mail |
[email protected] |
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| Significant scientific contributions: |
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Identified and characterized two novel cancer-testis antigens (CTA) as cancer biomarkers in Cervical Carcinoma: CABYR and PP1g2 (DBT Grant number BT/PR15312/MED/30/601/2011). |
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Standardized a protocol for stripping and re-probing of Western blots developed with Colorimetric substrate TMB (Electrophoresis 2012: 33: 1-4) |
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Characterized the expression of PP1g2 in MII arrested Mouse Oocytes and established its key role in MII Exit (Manuscript communicated to PLoS One). Also established the role of the protein as a motility marker for human sperms based on analysis of clinical samples. |
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Demonstrated Spatio-Temporal Redistribution of PP1γ2 in Actively Dividing HeLa cells (under communication) |
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Established a rodent model for the characterization of placental breach through a HIV-1 Nef (PLoS One 2012). |
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Standardized mouse IVF for high-throughput screening of fertilization inhibitors and identified natural compounds for anti-implantation female contraceptives. |
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Identified and characterized a novel egg receptor SAS1B for a sperm ligand SLLP1 and developed knock out mice for the same as well as targeted for female contraception (Patent # PCT/US2009/063540; Biology of Reproduction 2008: 78: 69: 72 & Developmental Biology 2012: 363: 40-51). |
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Characterized two novel mammalian oocyte pre-patterning marker proteins Ecat1 & HOEP19 and a pre-patterning locus in humans at chromosome number 6 during early embryonic development (Patent # PCT/US2006/005970; Biology of Reproduction 2008: 78: 163: 463). |
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Established a specific isoform of Glutathione S-transferases (GST) as a prognostic as well as diagnostic marker for breast cancer (Clinical Biochemistry 2005: 38: 436-443). |
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Developed a strategy for conservation of wild cats (including lions and tigers) through selective breeding and also identified putative sperm donors based on their MHC polymorphism (Tissue Antigens 2005: 66: 9-18). |
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Identified species specific microsatellite markers for wild cats. |
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Developed a telomere specific marker of Leishmania for the early diagnosis of Leishmaniasis (Am J Trop Med Hyg 2001: 65(5):573-82). |
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Established ‘Taxol’ as a novel drug for treating Leishmaniasis (FEMS Microbiol. Lett. 1999: 176: 429-435). |
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Identified inhibitors for Leishmania specific Glutathione synthesis; as chemotherapeutic targets (Trop. Med. Int. Health. 2000: 5(6): 438-442). |
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| Techniques known: |
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Molecular Biology: All the routine techniques related to handling DNA, RNA and proteins and their expression analysis
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Immunology: Raising of Polyclonal Antibodies, Direct Agglutination Test, Immunodiffusion techniques, 2D Proteomics, Immuno-blotting, Immunoprecipitation, ELISA
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Biochemistry: HPLC, TLC, ODC assay, Polyamine assay and Drug studies on Leishmanial growth
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Cell Culture: Mammalian and Animal cell line culture, transgenic animal development, Leishmania Cell Culture (in-vivo & in-vitro), Bacterial Cell Culture, Plant Tissue Culture, Follicle culture and egg collection, sperm swim-up along with IVF & ICSI in Mouse as well as humans
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Software: Autoassembler, Gene Tool II, Pep Tool I, Clustal, Phylip, Genedoc, Swiss prot, Arlequin, Setor and most of the other software commonly used for bioinformatics analyses |
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| Awards & Achievements: |
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Department of Biotechnology (DBT), Govt. of India Fellowship during M.Sc. Programme. |
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Qualified GATE examination (1996), conducted by IIT with a percentile score of 97.94 in Life Sciences. |
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Qualified ARS-NET (1997) in ‘Animal Biotechnology’, conducted by Indian Council of Agriculture Research |
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Qualified CSIR-NET (1999) in ‘Life Sciences’, conducted by Council of Scientific and Industrial Research. |
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Fogarty Post-doctoral Fellowship from NIH-USA for a Research Program in Population and Health in April 2004. |
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LEMTTF Award in 41st Meeting of SSR (Society for the Study of Reproduction), Kailua-Kona, Hawaii, USA. |
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Prof. KR Laumas Memorial Award in 18th Congress of Society of Andrology, in December 2012, Lucknow, India. |
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Three Best Poster Awards. |
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Selected on merit basis for six international trainings:
- Winter School of Immunology organized by Cancer Research Institute, New York, U.S.A. and the Ludwig Institute for Cancer Research New York, U.S.A.
- Frontiers in Reproduction organized by Marine Biological Laboratory, Woods Hole, Massachusetts, USA.
- Transgenic Technology and Gene Targeting organized by University of Virginia.
- Developing and propagating NIH-approved human embryonic stem (hES) cell lines as well as Induced differentiation of hES cells organized by Virginia Commonwealth University.
- Assisted Reproductive Technology organized by Embryology Academy of Research, Mumbai, India & Center for Reproductive Medicine, Cleveland Clinic, USA.
- Embryonic and Spermatogonial Stem Cell Biology organized by NDRI, Karnal, India.
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| Important Publications: |
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Corresponding Author: |
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• A protocol for stripping and re-probing of Western blots developed with Colorimetric substrate TMB
Electrophoresis 2012: 33: 1-4 |
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• HIV-1 Nef breaches placental barrier in Rat model.
PLoS One 2012: 7 (12): e51518 |
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• Tarun Barbhuiyan
(JRF) |
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• Shyam Sundar
Pal China (JRF) |
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• Monika Mittal
(JRF) |
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First Author: |
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• Oocyte specific oolemmal SAS1B involved in sperm binding through intra-acrosomal SLLP1 during fertilization Developmental Biology 2012: 363: 40-51 |
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• Mammalian RNA Binding Proteins ECAT1 and HOEP19 Reveal Oocyte Prepatterning Locus. Biology of Reproduction 2008: 78: 163: 463 |
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• Major Histocompatibility Complex class I polymorphism in Asiatic lions. Tissue Antigens 2005: 66: 9-18 |
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Co-Author: |
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• Identification of an Oolemmal Receptor for the Sperm Acrosomal Ligand, SLLP1 Biology of Reproduction 2008: 78: 69: 72 |
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• Evidence for the association of synaptotagmin with GST: Implications for a novel function in breast cancer.Clinical Biochemistry 2005: 38: 436-443 |
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• Conservation of wild animals by assisted reproduction and molecular marker technology Indian J Exp Biol. 2003 Jul; 41(7):710-23 |
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• Detection of Leishmania causing visceral leishmaniasis through PCR assay based on telomeric sequences. Am J Trop Med Hyg 2001 Nov; 65(5):573-82
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• Inhibition of glutathione synthesis as a chemotherapeutic strategy for Leishmaniasis Trop. Med. Int. Health. 2000 Jun; 5(6): 438-442 |
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• Antileishmanial effect of taxol, a microtubule stabilizing agent and its mechanism of action. FEMS Microbiol. Lett., 1999: 176: 429-435 |
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Book Chapter:
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Immunological considerations in conservation of wild animals You deserve, we conserve: A biotechnological approach to wildlife conservation: I. K. International Publishing House |
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Patents: |
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http://www.wipo.int/pctdb/en/ : (i) PCT/US2009/063540 and (ii) PCT/US2006/005970 |
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