| |
| |
| |
| |
| |
|
DR. SIDHARTH CHOPRA |
|
| |
|
.. |
Senior Scientist
Micobiology Division
Central Drug Research Institute
Lucknow - 226 031, India
|
| |
| Educational Qualifications |
Ph.D. in Microbiology / Biotechnology 2000 - 2004
International Centre for Genetic Engineering and Biotechnology
(ICGEB), New Delhi, India
(Dr. Anand Ranganathan - Advisor)
M.Sc. Microbiology- 1997 - 1999-
Panjab University, Chandigarh, India
B.Sc. Microbiology- 1994 -1997-
University of Delhi, New Delhi, India |
| Date of Birth |
09.02.1976 |
| E-Mail |
[email protected] , [email protected] |
|
| |
| WORK EXPERIENCE |
• |
Jan 2013-present |
Senior Scientist at CSIR-CDRI, Lucknow
I am presently engaged in Drug discovery and development against M. tuberculosis and several drug resistant gram-negative pathogens using natural and synthetic products. |
| • |
May 2012- Dec 2012 |
Scientist fellow at CSIR-CIMAP, Lucknow
I am presently engaged in Drug discovery and development against M. tuberculosis and several drug resistant gram-negative pathogens using natural and synthetic products. I am additionally trying to understand the genetic and proteomic basis of antimicrobial resistance in bacteria. |
• |
2008- 2012 |
Research Scientist at Centre for Infectious disease and Biodefense Research, Stanford Research Institute International, USA |
• |
2004 - 2008 |
Postdoctoral fellow
Microbiology and Immunology, Stanford University School of Medicine, USA (Professor Gary K. Schoolnik, Advisor) |
|
| |
| AREAS OF INTEREST |
• |
Microbiology with focus on Infectious diseases |
• |
Special emphasis on Mycobacterium species including non-tubercular mycobacteria, including gene knockouts, over-expression, transposon mutagenesis, TRASH analysis, antimicrobial susceptibility determination using MGIT |
• |
Drug discovery and development against MDR gram-positive and gram-negative bacteria including Acinetobacter baumannii, Psuedomonas sp, Klebsiella sp, MRSA etc. |
| • |
Cloning techniques, site directed mutagenesis, microarray design, data analysis & PCR based technologies |
• |
Protein expression and purification/interaction/bacterial two-hybrid system |
| • |
Microbial physiology and basic biochemistry including enzyme activity assays |
|
| |
| RESPONSIBILITIES INCLUDED |
|
• |
Project leader of anti-tuberculosis drug screening National Institutes of Health (NIH) contract |
• |
Understanding the mechanisms of antibiotic resistance generation in M. tuberculosis and A. baumannii |
• |
Drug-repurposing efforts to identify new indications for FDA-approved drugs against multi-drug resistant pathogens such as A. baumannii, M. abscessus and M. chelonae. |
• |
Developing low cost, non invasive novel diagnostics for detection of Mycobacterium tuberculosis |
• |
Identification of mycobacterium proteasome inhibitors using a novel luciferase assay |
• |
Microbial physiology and basic biochemistry including enzyme activity assays |
|
| |
| PEER REVIEWED PUBLICATIONS |
• |
Kashyap DR, Vohra PK, Chopra Sidharth, Tewari R. Applications of pectinases in the commercial sector: A Review. Bioresource Technology. 2001 May; 77(3): 215-27. Citation Index: 342.
Impact factor of Journal: 4.72 |
• |
Chopra Sidharth, Singh SK, Sati SP, Ranganathan A, Sharma A. Expression, purification, crystallization and preliminary X-ray analysis of the acyl carrier protein synthase (acpS) from Mycobacterium tuberculosis. Acta Crystallographica (D) Biological Crystallography. 2002 Jan; 58 (Pt 1): 179-81.Citation Index: 5.
Impact factor of Journal: 2.9 |
• |
Chopra Sidharth, Pai H, Ranganathan A. Expression, purification, and biochemical characterization of Mycobacterium tuberculosis aspartate decarboxylase, PanD. Protein Expression & Purification. 2002 Aug; 25(3): 533-40.Citation Index: 18.
Impact factor of Journal: 1.56 |
• |
Chopra Sidharth, Ranganathan A. Protein evolution by "codon shuffling": a novel method for generating highly variant mutant libraries by assembly of hexamer DNA duplexes. Chemistry & Biology. 2003 Oct; 10 (10): 917-26.Citation Index: 20.
Impact factor of Journal: 6.37 |
• |
Rao A, Chopra Sidharth*, Ram G, Gupta A, Ranganathan A. Application of the "codon-shuffling" method. Synthesis and selection of de novo proteins as antibacterials. Journal of Biological Chemistry. 2005 Jun 24; 280 (25): 23605-14. (* Equal contribution)
Citation Index: 9.
Impact factor of Journal: 7.38 |
• |
Gopalan G, Chopra Sidharth, Ranganathan A, Swaminathan K. Crystal structure of uncleaved L-aspartate-alpha-decarboxylase from Mycobacterium tuberculosis. Proteins. 2006, 65(4): 796-802.
Citation Index: 7.
Impact factor of Journal: 3.08 |
• |
Flores-Valdez and Sidharth Chopra. “Global reemergence of tuberculosis: Are host defense peptides an option to ameliorate disease burden?” Microbial drug resistance, 2010 Mar; 16(1):1-7.
Citation Index:
Impact factor of Journal: 1.99 |
• |
Chopra S, Torres-Ortiz M, Hokama L, Madrid P, Tanga M, Mortelmans K, Kodukula K, Galande AK. Repurposing FDA-approved drugs to combat drug-resistant Acinetobacter baumannii. J Antimicrob Chemother. 2010 Dec; 65(12):2598-601.
Citation Index: 3.
Impact factor of Journal: 4.65 |
• |
Sidharth Chopra*, Matsuyama K, Hutson K and Madrid P. Identification of antimicrobial activities amongst FDA-approved drugs for combating Mycobacterium abscessus and Mycobacterium chelonae. J Antimicrob Chemother. 2011 Jul; 66(7): 1533-6 (*First and corresponding author)
Citation Index: 3.
Impact factor of Journal: 4.65 |
• |
Malabika Sarker, Sidharth Chopra, Kristien Mortelmans, Krishna Kodukula, Carolyn Talcott, and Amit K. Galande. In Silico Pathway Analysis Predicts Metabolites That Are Potential Antimicrobial Targets. Journal of Computer Science and System Biology. (DOI http://dx.doi.org/10.4172/jcsb.1000071)
Citation Index: 5.
Impact factor of Journal: 2.8 |
• |
Arlyn Tambo-ong, Sidharth Chopra, Bryan T. Glaser, Karen Matsuyama, Tran Tran and Peter B. Madrid. Mannich reaction derivatives of novobiocin with modulated physiochemical properties and their antibacterial activities. Bioorg Med Chem Lett. Oct 1; 21(19): 5697-700.
Impact factor of Journal: 2.7 |
• |
Sidharth Chopra* and Amit Galande. A Fluoroquinolone-resistant Acinetobacter baumannii without the Quinolone Resistance-Determining Region Mutations. Journal of Antimicrobial Chemotherapy, Nov 2011; 66(11): 2668-70. (* First and corresponding author).
Impact factor of Journal: 4.65 |
• |
Sidharth Chopra; Matsuyama, Karen; Tran, Tran; Malerich, Jeremiah; Lun, Shichun; Guo, Haidan; Maiga, Miriama; Wan, Baojie; Franzblau, Scott; Bishai, William; Madrid, Peter. Gyrase B as validated drug target in Mycobacterium tuberculosis. J Antimicrob Chemother. 2012 Feb; 67(2): 415-21.Impact factor of Journal: 4.65 |
• |
Malabika Sarker, Carolyn Talcott, Peter Madrid, Sidharth Chopra, Barry A. Bunin, Gyanu Lamichhane, Joel S. Freundlich and Sean Ekins. Combining Cheminformatics Methods and Pathway Analysis to Identify Molecules with Whole-Cell Activity against Mycobacterium tuberculosis. Pharm Res. 2012, Aug; 29(8):2115-27.
Impact factor of Journal: 4.65 |
• |
Sidharth Chopra, Koolpe GA, Tambo-Ong AA, Matsuyama KN, Ryan KJ, Tran TB, Doppalapudi RS, Riccio ES, Iyer LV, Green CE, Wan B, Franzblau SG, Madrid PB. Discovery and Optimization of Benzotriazine Di-N-Oxides Targeting Replicating and Non-replicating Mycobacterium tuberculosis. J Med Chem. 2012 Jul 12; 55(13): 6047-60.Citation Index:
Impact factor of Journal: 5.2 |
• |
Chopra, Sidharth; Matsuyama, Karen; Tran, Tran; Madrid, Peter. Systematic discovery of synergistic novel antibiotic combinations targeting multidrug resistant Acinetobacter baumannii. International Journal of Antimicrobial Agents, 2012. Oct; 40(4): 377-9.
Impact factor of Journal: 3.8 |
• |
Kurnellas MP, Brownell SE, Su L, Malkovskiy AV, Rajadas J, Dolganov G, Chopra Sidharth, Schoolnik GK, Sobel RA, Webster J, Ousman SS, Becker RA, Steinman L, Rothbard JB. Chaperone activity of small heat shock proteins underlies therapeutic efficacy in experimental autoimmune Encephalomyelitis. J Biol Chem. 2012 Sep 6.
Citation Index Impact factor of Journal: 4.8 |
• |
Madrid PB, Chopra S, Manger ID, Gilfillan L, Keepers TR, Shurtleff AC, Green CE, Iyer LV, Dilks HH, Davey RA, Kolokoltsov AA, Carrion R Jr, Patterson JL, Bavari S, Panchal RG, Warren TK, Wells JB, Moos WH, Burke RL, Tanga MJ.A systematic screen of FDA-approved drugs for inhibitors of biological threat agents. PLoS One. 2013;8(4):e60579. doi: 10.1371/journal.pone.0060579. |
• |
Chopra S, Ramkissoon K, Anderson DC.A systematic quantitative proteomic examination of multidrug resistance in Acinetobacterbaumannii.J Proteomics. 2013 Jun 12;84:17-39. |
• |
Kumar K, Chopra S.New drugs for methicillin-resistant Staphylococcus aureus: an update.J AntimicrobChemother. 2013 Jul;68(7):1465-70. |
| |
| Patents |
• |
US patent:
P Madrid, S Chopra, K Ryan, G Koolpe - US Patent 20,130,150,369, 2013. Benzotriazine Oxides as Drugs Targeting Mycobacterium Tuberculosis |
• |
International patents: Inventors: Hans-Henrik Kristensen Hogenhaug Gary K. Schoolnik, Sidharth Chopra and Dorotea Raventos Segura .
Title: USE OF DEFENSINS AGAINST TUBERCULOSIS
U.S. patent application no. PCT/EP2009/052405 |
• |
Indian patents: Inventors: Ranganathan, Anand, Chopra, Sidharth Title: Conscious evolution of proteins.
Priority Date: 16 April 2003.
Indian Patent Application no. 609/DEL/2003 |
|
| |
| PEER REVIEW OF MANUSCRIPTS FROM |
• |
Biomed Central Microbiology |
• |
Journal of Antimicrobial Chemotherapy |
• |
Drug Discovery Today |
• |
PLoS One |
• |
Journal of Infectious diseases |
• |
PLoS Medicine |
• |
International Journal of Tuberculosis and Lung Disease |
• |
Molecular Microbiology |
• |
Journal of Clinical Microbiology |
• |
Antimicrobial and Agents Chemotherapy |
• |
Journal of Bacteriology |
|
| |
| CONFERENCES AND POSTERS |
• |
Sidharth Chopra and Anand Ranganathan. “Studies on L-Aspartate decarboxylase of Mycobacterium tuberculosis”. Poster presented at International Congress on Infectious diseases (ICID) held from March 11-14th, 2002 in Singapore. |
• |
Sidharth Chopra and Anand Ranganathan. “L-Aspartate decarboxylase of Mycobacterium tuberculosis:a validated drug and vaccine target”. Poster presented at the First International Conference on “TB Vaccines for the World” held from 17-19th of September 2003 in Montreal, Canada. |
• |
Gopalan Gayathri, Sidharth Chopra, Anand Ranganathan & K. Swaminathan. “Structural investigations of L-Aspartate decarboxylase from Mycobacterium tuberculosis”. Poster presented at Third International Conference on Structural Biology and Functional Genomics, Singapore, December 2-4, 2004. |
• |
Alka Rao, Sidharth Chopra, Geeta Ram, Anand Ranganathan. “Synthesis And Selection of Antibacterials using Codon-Shuffling As Method For De Novo Protein Design”. Poster presented at 14th International Conference on Intelligent Systems for Molecular Biology, Fortaleza, Brazil, August 6-10, 2006. |
|
|
| |
|
|
| |
|
| |
|