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ASHISH ARORA |
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Senior Scientist
Molecular & Structural Biology Division |
| E- Mail |
ashishcdri[at]yahoo
dot com |
| Educational Qualifications |
PhD in Biotechnology from
Panjab University (1996), MSc in Organic Chemistry from
Rajasthan University (1990) |
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| CURRENT
AREAS OF INTEREST |
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Cloning, Expression
and Purification of M. tuberculosis proteins. |
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NMR Spectroscopy
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Fluorescence
and CD spectroscopy |
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Structural
Genomics of Mycobacterium tuberculosis Proteins by NMR
Spectroscopy |
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Structural Genomics of Mycobacterium tuberculosis Proteins by
NMR Spectroscopy |
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| Our
aim is to clone, express and isotopically label important
protein drug targets of Mycobacterium tuberculosis,
for determination of their structures by NMR spectroscopy,
and use the structural information in subsequent high-throughput
screening for drug development. Currently, I am directly
supervising three graduate (Ph.D.) students. I am also
involved in planning the NMR facility for protein structure
determination at CDRI, Lucknow. We are also interested
in studying the thermodynamic stability of protein and
protein-ligand complexes using a variety of biophysical
methods. |
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| RECENT
PUBLICATIONS |
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Luthra A, Mahmood A, Arora
A and Ravishankar Ramachandran - Characterization
of Rv3868: an essential hypothetical protein of the ESX-1
secretion system in M. tuberculosis (2008) - J.
Biological Chemistrty 283, 36532-36541 |
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Pulavarti SV, Jain A,
Pathak PP, Mahmood A, Arora A. - Solution
structure and dynamics of peptidyl-tRNA hydrolase from
Mycobacterium tuberculosis H37Rv. (2008) - J Mol
Biol. 378,165-77 |
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Agrawal H, Kumar A, Bal
NC, Siddiqi MI and Arora A. - Ligand
based virtual screening and biological evaluation of inhibitors
of chorismate mutase (Rv1885c) from Mycobacterium tuberculosis
H37Rv. (2007) - Bioorganic & Medicinal Chemistry
Letters 17, 3053-3058 |
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Bal NC, Agrawal H, Meher
AK and Arora A. - Characterization of
peptidyl-tRNA hydrolase encoded by open reading frame
Rv1014c of Mycobacterium tuberculosis H37Rv. (2007)
- Biological Chemistry 388, 467-479 |
• |
Gupta Prasoon, Akanksha,
Siripurapu Kiran Babu, Ahmad Ausaf, Palit Gautam, Arora
Ashish and Maurya Rakesh. - Anti-stress constituents
of Evolvulus alsinoides: An ayurvedic crude drug. (2007)
- Chem. Pharm. Bull 55, 771-775 |
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Meher Akshaya K, Lella
Ravi K, Sharma Charu and Arora Ashish.
- Analysis of complex formation and immune response of
CFP-10 and ESAT-6 mutants. (2007) - Vaccine
25, 6098-6106 |
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Yadav Prem P, Arora
Ashish, Bid Hemant K, Konwar Ritu R and Kanojiya
Sanjeev - New cassane butenolide hemiketal diterpenes
from the marine creeper Caesalpinia bonduc and their antiproliferative
activity. (2007) - Tetrahedron Letters
48, 7194-7198 |
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Bal, N C, Agrawal, H,
Meher, A K, Pulavarti, S V, Jain, A, Kelly, G, Frenkiel,
TA, Pastore, A, and Arora, A. - NMR assignment
of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis
H37Rv. Medicinal Chemistry of ureides as antiinfectives
(2006) - Journal Of Biomolecular NMR,
36, 53. |
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Meher, Akshaya K; Bal,
Naresh Chandra; Chary, Kandala V R; Arora, Ashish
- Mycobacterium tuberculosis H37Rv ESAT-6-CFP-10 complex
formation confers thermodynamic and biochemical stability
(2006) - FEBS Journal, 273,1445-1462.
( European Journal of Biochemistry ), Volume 273, Issue
7, , April 2006, 1445-1462. |
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Tamm, L.K., Abildgaard,
F., Arora, A., Blad, H. & Bushweller,
J.H. - Structure, dynamics and function of the outer membrane
protein A (OmpA) and influenza hemagglutinin fusion domain
in detergent micelles by solution NMR. (2004)
- FEBS Letters, 555, 139-53. |
| • |
Ashish
Arora, I. M. Williamson, A. G. Lee, and D. Marsh
Lipid-protein interactions with cardiac phospholamban
studied by spin-label electron spin resonance.Biochemistry
(2003), 42, 5151-8. |
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Ashish
Arora and L. K. Tamm, Biophysical approaches
for membrane protein structure determination.
Curr. Opin. Struct. Biol. (2001) 11, 540-547. |
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L.K. Tamm,
Ashish Arora and J.H. Kleinschmidt ,
Structure and assembly of -barrel membrane proteins. J.
Biol. Chem. (2001), 276(35), 32399-402 |
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Ashish
Arora, F. Abildgaard, J.H. Bushweller and L.K.
Tamm, Structure of the outer membrane protein A transmembrane
domain by NMR spectroscopy. Nature Struct. Biol.
(2001), 8(4), 334-338. |
| • |
Ashish
Arora, D. Rinehart, G. Szabo and Lukas K. Tamm,
Refolded Outer membrane protein A of Escherichia coli
forms ion channels with two conductance states in planar
lipid bilayers. J. Biol. Chem. (2000)
275(3), 1594-1600. |
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