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DR. Wahajuddin |
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Scientist
Pharmacokinetics & Metabolism Division
CSIR- Central Drug Research Institute
Lucknow - 226031 |
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| RESEARCH
ACTIVITIES |
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Bio-analysis
Bio-analytical method development and validation, as per the regulatory (US-FDA, ICH etc.) guidelines, on HPLC and LC-MS/MS platform for quantitative determination of small molecules (NCEs/Drugs and/or metabolites) in different biological matrices |
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Pharmacokinetic studies
Characterization of the in vivo pharmacokinetics (bio-availability, dose-proportionality, gender variation, inter-species comparison) following oral and parenteral routes of administration in animal models. Toxicokinetic and tissue distribution studies to support regulatory toxicology |
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Metabolism, Metabolite Identification and CYP Phenotyping
In vitro/in vivo Metabolism of new chemical entities and/or drugs using animal models, scaling preclinical and in-vitro data to predict events in humans for preliminary assessment of safe exposure levels, determination of the metabolite profiles of drug candidates using liver microsomes/human recombinant CYP450 isozymes, Identify Phase I or Phase II enzymes responsible for metabolite formation, predict drug interactions due to inhibition or induction of drug metabolizing enzymes. |
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Interaction Studies
Drug-drug, herb-drug and food-drug interaction studies for suggesting benefits (as potential combination)/risks of the same in therapies. |
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Biopharmaceutics
Permeability/Absorption Characterization Studies across Parallel Artificial Membrane Permeability Assay (PAMPA) and in situ rat model, Protein Binding and Simulated Gastro-intestinal Stability for new chemical entities/drugs. |
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| HONOURS & AWARDS |
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Visiting Scientist- UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill (2013-14) |
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Young Pharmaceutical Analyst Award 2012 from Indian Drug Manufacturer’s Association
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DBT- Cutting Edge Research Enhancement & Scientific Training (CREST) Award 2011-12 from Dept. of Biotechnology, Govt. of India. |
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Selected for SERB-DST Fast Track Young Scientist Proposal Award 2011 from Dept. of Sc. & Tech. Govt. of India |
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Selected as TWAS/BVA.NXT2012 Fellow by Academy of Sciences in Developing World (TWAS) and Bibliotheca Alexandria Egypt |
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Won Prof. A. K. Dey Award 2011 for Analytical and Environmental Chemistry by Indian Chemical Society |
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Profile appeared in the international journal Bioanalysis as nominated Young Investigator 2011 (PMID: 21510757). |
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| HUMAN
RESOURCE DEVELOPMENT |
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Current PhD students: Mr KSR Raju (SRF), Ms Isha Taneja (SRF), Mr Mamunur Rshid (JRF) |
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Current Project Fellows: Mr Sandeep K Singh, Mr Mohd Yaseen Malik, Ms Swati Chaturvedi |
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Training provided to Post Graduates: 26 |
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| RECENT PUBLICATION
: Research Articles : 59 Patents: 5 Book Chapters: 5 Abstract:
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| 2015 |
| Corresponding author |
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1. Raju KSR, Taneja I, Valicherla GR, Challagundla MK, Gayen JR, Singh SP, Wahajuddin M§ (2015) No effect on pharmacokinetics of tamoxifen and 4-hydroxytamoxifen by multiple doses of red clover capsule in rats. Scientific Reports. (In Press) (§Corresponding author) (Impact Factor-5.578) |
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2. Taneja I, Raju KSR, Singh SP, Wahajuddin M§ (2015) Assessment of pharmacokinetic compatibility of short acting CDRI candidate trioxane derivative, 99-411, with long acting prescription antimalarials, lumefantrine and piperaquine. Scientific Reports. (In Press) (§Corresponding author) (Impact Factor-5.578) |
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3. Taneja I, Raju KSR and Wahajuddin M§ (2015) Dietary isoflavones as modulators of drug metabolizing enzymes and transporters: Effect on prescription medicines. Critical reviews in food science and nutrition (Accepted) (§Corresponding author) (Impact Factor -5.58) |
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4. Taneja I, Raju KSR, Mittal M, Dev K, Khan MF, Maurya R and Wahajuddin M§ (2015) Oral bioavailability, intravenous pharmacokinetic, plasma protein binding and metabolic stability studies of ALDH2 activator, alda-1, using validated LC-ESI-MS/MS method in rat plasma. RSC Advances. 5: 54395-54402 (Impact Factor-3.7) (§Corresponding author) |
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5. Wahajuddin M§, Singh SP, Taneja I, Raju KSR, Gayen JR, Siddiqui HH, Singh SK (2015) Development and validation of a LC-MS/MS method for simultaneous determination of piperaquine and 97-63, the active metabolite of CDRI 97-78, in rat plasma and its application in interaction study. Drug Testing and Analysis. (Accepted) doi: 10.1002/dta.1807. PMID: 25975936 (§Corresponding author) (Impact Factor -2.84) (§Corresponding author) |
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6. Arora S, Taneja I, Challagundla M, Raju KSR, Singh SP, Wahajuddin M§ (2015) In vivo prediction of CYP-mediated metabolic interaction potential of formononetin and biochanin A using in vitro human and rat CYP450 inhibition data. Toxicology Letters. 239(1):1-8. PMID: 26278343 (Impact Factor-3.3) (§Corresponding author) |
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7. Taneja I, Raju KSR, Challagundla M, Raghuvanshi A, Goel A, Wahajuddin M§ (2015) LC-ESI-MS/MS method for bioanalytical determination of osteogenic phytoalexin, medicarpin, and its application to preliminary pharmacokinetic studies in rats. J Chromatography B. 1001:9-16. PMID: 26241182 (Impact Factor-2.7) (§Corresponding author) |
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8. Wahajuddin M§, Singh SP, Taneja I, Raju KSR, Gayen JR, Siddiqui HH, Singh SK (2015) Simultaneous quantification of proposed anti-malarial combination comprising of lumefantrine and CDRI 97-78 in rat plasma using the HPLC–ESI-MS/MS method: Application to drug interaction study. Malaria J. 14(1):172. PMID: 25895956 (§Corresponding author) (Impact Factor – 3.49) |
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9. Raju KSR, Taneja I, Singh SP, Tripathi A, Mishra DP, Hussain KM, Gayen JR, Singh SK and Wahajuddin M§ (2015)Simultaneous determination of Centchroman and tamoxifen along with their metabolites in rat plasma using LC-MS/MS. Bioanalysis. 7(8):967-979. PMID: 25966009 (§Corresponding author) (Impact Factor-3.25) |
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10. Raju KS, Kadian N, Taneja I and Wahajuddin M§ (2015) Phytochemical analysis of isoflavonoids using liquid chromatography coupled with tandem mass spectrometry. Phytochemistry Reviews :1-30 (2015) §Corresponding author) (Impact Factor-2.89) |
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11. Dwivedi P, Khatik R, Chaturvedi P, Khandelwal K, Taneja I, Raju KS, Dwivedi H, Singh S, Gupta PK, Shukla P, Tripathi P, Singh S, Tripathi R, Wahajuddin, Paliwal SK, Dwivedi AK and Mishra PR (2015) Arteether nanoemulsion for enhanced efficacy against Plasmodium yoelii nigeriensis malaria: An approach by enhanced bioavailability. Colloid Surface B. 126:467-75 PMID 25616971 (Impact Factor -4.29) |
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12. Singh S, Chauhan P, Ravi M, Taneja I, Wahajuddin, Yadav PP (2015) A mild CuBr-NMO oxidative system for the coupling of anilines leading to aromatic azo compounds. RSC Advances. 5:61876-61880 (Impact Factor-3.7) |
| 2014 |
| Corresponding author |
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13. Wahajuddin§,Kanumuri Siva Rama Raju, Sheelendra Pratap Singh and Isha Taneja (2014) Investigation of Functional Role of P-glycoprotein in Limiting the Oral Bioavailability of Lumefantrine. Antimicrobial Agents and Chemotherapy. 58(1):489-494. PMID: 24189249 (§Corresponding author) (Impact Factor-4.5) |
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14. CaiH, WahajuddinM, EverettR, ThakkerDR. (2014) Do cation-selective transporters help or hurt the antitumor efficacy of metformin in breast cancer. Cancer Res. 74(19): 4639-4639. 2014. (Impact Factor-9.2) |
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15. Dwivedi P, Khatik R, Khandelwal K, Srivastava R, Taneja I, Raju KS,Dwivedi H, Shukla P, Gupta P, Singh S, Tripathi R, Paliwal SK, Wahajuddin, Dwivedi AK and Mishra PR. (2014) Self-nanoemulsifying drug delivery system (SNEDDS) for oral delivery of arteether: pharmacokinetics, toxicity and antimalarial activity in mice. RSC Advances. 4:64905-64918 (Impact Factor-3.7) |
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16. ChauhanP, RaviM, SinghS,RajuKS, BajpaiV, KumarB, Wahajuddin and Yadav PP. (2014) Palladium and copper-catalyzed ligand-free coupling of phenylhydrazines in water. RSC Advances. 4(82): 43336-43340. (Impact Factor-3.7) |
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17. Sonam Gupta; Dinesh K Verma; Joyshree Biswas; Sivaram K Raju; Neeraj Joshi; Wahajudin; Sarika Singh (2014) The metabolic enhancer piracetam attenuates the mitochondrion-specific endonuclease-G translocation and oxidative DNA fragmentation. Free Radical Biology & Medicine. 73C:278-290.(Impact Factor-5.3) |
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18. Dwivedi P, Khatik R, Khandelwal K, Taneja I, Raju KS, Wahajuddin,/b>, Paliwal SK, Dwivedi AK, Mishra PR. (2014) Pharmacokinetics study of arteether loaded solid lipid nanoparticles: An improved oral bioavailability in rats.International Journal of Pharmaceutics. 466(1-2):321-327.PMID: 24657144 |
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19. Tripathi A, Singh SP, Raju KSR, Wahajuddin and Gayen JR (2014) Effect of Red Clover on CYP Expression: An Investigation of Herb-Drug Interaction at Molecular Level. Indian Journal of Pharmaceutical Sciences. 76(3): 261-266. |
| 2013 |
| Corresponding author |
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20. Isha Taneja, Madhuri Erukala, Kanumuri Siva Rama Raju, Sheelendra Pratap Singh and Wahajuddin§ (2013) Dried Blood Spots in Bioanalysis of Antimalarials: Relevance and Challenges in Quantitative Assessment of Antimalarial Drugs. Bioanalysis. 5(17):2171-86. PMID: 23962252(§Corresponding author) (Impact Factor-3.2) |
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21. Kanumuri Siva Rama Raju, Isha Taneja, Sheelendra Pratap Singh and Wahajuddin§ (2013) Utility of non-invasive biomatrices in pharmacokinetic studies. Biomedical Chromatography27(10):1354-66.PMID: 23939915.(§Corresponding author) (Impact Factor-1.9) |
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22. Wahajuddin§, Kanumuri Siva Rama Raju, Isha Taneja (2013) Bioanalysis of antimalarials using liquid chromatography. TrAC Trends in Analytical Chemistry. 42: 186-204. (§Corresponding author) (Impact Factor-6.273) |
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23. Wahajuddin§, Isha Taneja, Sumit Arora, Kanumuri Siva Rama Raju, Nida Siddiqui (2013) Disposition of Pharmacologically Active Dietary Isoflavones in Biological Systems. Current Drug Metabolism. 14(4):369-80. PMID: 23330931 (§Corresponding author) (Impact Factor-5.113) |
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24. Wahajuddin, Kanumuri Raju, Isha Taneja, Amit Tripathi, Durga Mishra (2013) “Drug-Drug Interaction Potential Assessment of Tamoxifen and Centchroman”AAPS Journal. W4275
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25. Srivastava K, Tyagi AM, Khan K, Dixit M, Lahiri S, Kumar A, Changkija B, Khan MP, Nagar GK, Yadav DK, Maurya R, Singh SP, Jain GK, Wahajuddin, Trivedi R, Chattopadhyay N, Singh D. (2013) Isoformononetin, a methoxydaidzein present in medicinal plants, reverses bone loss in osteopenic rats and exerts bone anabolic action by preventing osteoblast apoptosis. Phytomedicine. 20(6):470-480. PMID: 23395215 (Impact Factor-3.268) |
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26. Narender Tadigoppula, Venkateswarlu Korthikunta, Shweta Gupta, Papireddy Kancharla, Tanvir Khaliq, Awakash Soni, Rajeev Kumar Srivastava, Kumkum Srivastava, Sunil Kumar Puri, Kanumuri Siva Rama Raju, Wahajuddin, Puran Singh Sijwali, Vikash Kumar, and Imran Siddiqi Mohammad (2013) Synthesis and Insight into the Structure–Activity Relationships of Chalcones as Antimalarial Agents. J. Med. Chem., 56(1):31-45. PMID: 23270565(Impact Factor-5.248) |
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27. Ranjani Maurya, Awakash Soni, Devireddy Anand, Makthala Ravi, Kanumuri S. R. Raju, Isha Taneja, Niraj K. Naikade, S. K. Puri, Wahajuddin, Sanjeev Kanojiya, and Prem P. Yadav (2013) Synthesis and Antimalarial Activity of 3,3-Spiroanellated 5,6-Disubstituted 1,2,4-Trioxanes. ACS Med. Chem. Lett., 4(2): 165–169.(Impact Factor-3.355) |
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28. Hao Cai, Wahajuddin, Ravindra Alluri, Ruth Everett, Dhiren Thakker(2013) “Cation-Selective Transporters are Critical Determinants of the Anti-proliferative Effects of Metformin in Breast Cancer Cells” AAPS Journal.W4368 |
| 2012 |
| Corresponding author |
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29. Wahajuddin§ and Sumit Arora (2012) Superparamagnetic iron oxide nanoparticles: Magnetic nanoplatforms as drug carriers. International Journal of Nanomedicine,2012(7): 3445-3471. PMID:22848170 (§Corresponding author) (Impact Factor-4.976) |
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30. Wahajuddin§, Sheelendra Pratap Singh, KSR Raju, Nafis A and Girish Kumar Jain (2012) Simultaneous determination of nine model compounds in permeability samples using RP-HPLC: Application to prove the cassette administration principle in single pass intestinal perfusion study in rats. Journal of Pharmaceutical and Biomedical Analysis.67-68:71-76. PMID: 22613582 (§Corresponding author) (Impact Factor- 2.733) |
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31. Wahajuddin§, Sheelendra Pratap Singh and Girish Kumar Jain (2012) Gender differences in pharmacokinetics of lumefantrine and its metabolite desbutyl-lumefantrine in rats. Biopharm Drug Disposition. 33(4):229-34. PMID: 22447530 (§Corresponding author) (Impact Factor-1.394) |
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32. Sheelendra Pratap Singh*, Wahajuddin*§, KSR Raju, Mushir M. Ali, Kanchan Kohli, Girish Kumar Jain (2012) Reduced bioavailability of tamoxifen and its metabolite 4-hydroxytamoxifen after oral administration with biochanin A (an isoflavone) in rats. Phytotherapy Research.26(2):303-7. PMID: 22131128 (*Authors contributed equally; §Corresponding author)(Impact Factor-1.81) |
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33. Vikram Khedgikar, Jyoti Gautam, Priyanka Kushwaha , Avinash Kumar, Geet K. Nagar, Preety Dixit, Raju Chillara, Swathi Voruganti, Sheelendra P. Singh, Wahaj Uddin, Girish K. Jain, Divya Singh, Rakesh Maurya, Naibedya Chattopadhyay, Ritu Trivedi (2012) A standardized phytopreparation from an Indian medicinal plant (Dalbergia sissoo) has antiresorptive and bone-forming effects on a postmenopausal osteoporosis model of rat. Menopause 2012; 19(12): 1. PMID: 22850441 (Impact Factor-3.318) |
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34. Chakraborty J, Hossain MZ, Tripathi A, Ghosh W, Samanta TB, Wahajuddin and Gayen JR (2012) Taxonomical characterization of a penicillin amidase producing novel strain of Achromobacter xylosoxidans: Proposal of A. xylosoxidans subsp. indiges subsp. nov. Developmental Microbiology and Molecular Biology. 3(1): 55-65. (Impact Factor) |
| 2011 |
| Corresponding author |
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35. Wahajuddin§, Sheelendra Pratap Singh, Sunil Kumar Puri and Girish Kumar Jain (2011) Intravenous pharmacokinetics, oral bioavailability, dose proportionality and in situ permeability of anti-malarial lumefantrine in rats. Malaria Journal,10(1):293PMID: 21985153 (§Corresponding author) (Impact Factor-3.49) |
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36. Sheelendra Pratap Singh*, Wahajuddin*§, Divyesh Tewari1, Kushalkumar Patel, Girish Kumar Jain (2011) Permeability determination and pharmacokinetic study of nobiletin in rat plasma and brain by validated high-performance liquid chromatography method. Fitoterapia 82 (8): 1206-14. PMID: 21888953 (*Authors contributed equally; §Corresponding author) (Impact Factor-1.81) |
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37. Sheelendra Pratap Singh*, Wahajuddin*§, Mushir M. Ali, Kanchan Kohli, Girish Kumar Jain (2011) Liquid chromatography–mass spectrometry method for the quantification of tamoxifen and its metabolite 4-hydroxytamoxifen in rat plasma: Application to interaction study with biochanin A (an isoflavone). Journal of Chromatgraphy B.879(27): 2845-2851.PMID: 21890435 (*Authors contributed equally; §Corresponding author) (Impact Factor-2.97) |
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38. Singh SP*, Wahajuddin*§ and Jain GK (2011) Intravenous Pharmacokinetics and Oral Bioavailability of Biochanin A in Rats. Medicinal Chemistry Research, 20(9-S1): 16271631.(*Authors contributed equally; §Corresponding author) (Impact Factor-1.1) |
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39. Wahajuddin§, Sheelendra Pratap Singh, Kushal Patel, Tejaswini Pradhan, Hefazat Husain Siddiqui, Shio Kumar Singh (2011) Prediction of human absorption of trioxane antimalarial (99-411) using in-house validated in-situ single-pass intestinal perfusion model. Arzneimittel-Forschung/Drug Research. 61(10): 1-6 PMID: 22029231(§Corresponding author) (Impact Factor-1) |
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40. Wahajuddin (2011) Young Investigator: Wahajuddin. Bioanalysis. 3(8): 839-841. PMID: 21510757 (Impact Factor-3.34) |
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41. Singh SP*, Wahajuddin*§, Tewari D, Pradhan T and Jain GK (2011) PAMPA permeability, plasma protein binding, blood Partition, pharmacokinetics and metabolism of formononetin, a methoxylated isoflavone. Food and Chemical Toxicology. 49(5):1056-62. PMID: 21266188, (*Authors contributed equally; §Corresponding author) (Impact Factor-2.60) |
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42. Wahajuddin§, Singh SP, Singh SK and Jain GK (2011) Quantitation of Lumefantrine in biological matrices. Curr. Pharm. Anal. 7(1): 42-46.(§Corresponding author)(Impact Factor-1.71) |
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43. Sarika Singh, Poonam Goswami, Supriya Swarnkar, Sheelendra P Singh, Wahajuddin, Chandishwar Nath, Sharad Sharma (2011) A Study to evaluate the effect of nootropic drug - piracetam on DNA damage in leukocytes and macrophages. Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 726(1):66-74. PMID: 21920458 (Impact Factor-2.938) |
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44. Suryawanshi S, Wahajuddin, Gupta RC and Singh SK (2011) Preclinical Pharmacokinetics, Dose Proportionality, Gender Difference and Protein Binding Study of 16-Dehydropregnenolone, an Antihyperlipidemic Agent, in Rats. Journal of Pharm. and Pharmacol. 63(1):41-8. PMID:21155814(Impact Factor-1.91) |
| 2010 |
| Corresponding author |
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45. Wahajuddin§, Sheelendra Pratap Singh* and Girish Kumar Jain (2010) “Pharmacokinetics and Bioavailability of Lumefantrine, a Highly Protein Bound Antimalarial in Rats” AAPS Journal. 12(S2): W4374. (§Corresponding author) (Impact Factor-3.8) |
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46. Sheelendra Pratap Singh*, Wahajuddin*§ and Girish Kumar Jain (2010) “Simultaneous determination of four isoflavones in rat plasma by LC-ESI-MS/MS: Assay development, validation and application to pharmacokinetic study” AAPS Journal. 12(S2): T2313. (*Authors contributed equally; §Corresponding author) (Impact Factor-3.8) |
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47. Singh SP*, Wahajuddin*§, Ali MM, Jain GK (2010) Determination and pharmacokinetic study of biochanin A and its metabolite genistein in rat plasma by liquid chromatography–mass spectrometry. Journal of Separation Science, 33 (21): 3326-34. PMID: 21049521(Impact Factor-2.63)(*Authors contributed equally; §Corresponding author) |
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48. Singh SP*, Wahajuddin*§, Jain GK.,(2010)Determination of curcumin in rat plasma by liquid–liquid extraction using LC–MS/MS with electrospray ionization: Assay development, validation and application to a pharmacokinetic study. Journal of Bioanalysis & Biomedicine. 2(4): 79-84.(*Authors contributed equally; §Corresponding author) |
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49. Singh SP*, Wahajuddin*, Yadav DK, Rawat P, Maurya R, Jain GK (2010) Quantitative determination of formononetin and its metabolite in rat plasma after intravenous bolus administration by HPLC coupled with tandem mass spectrometry. Journal of Chromatography B. 878 (3-4): 391-7. PMID: 20034859 (Impact Factor-2.78) (*Authors contributed equally) |
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50. Abnish K. Gautam, Biju Bhargavan, Abdul M. Tyagi, Kamini Srivastava, Dinesh K. Yadav, Manmeet Kumar, Akanksha, Jay S. Mishra, Amar Bahadur Singh, Sabyasachi Sanyal, Rakesh Maurya, Lakshmi Manickavasagam, Sheelendra P. Singh, Wahajuddin, Girish K. Jain, Naibedya Chattopadhyay, Divya Singh (2011) Effects of Mathoxylated Daidzeins, Cladrin and Formononetin on Osteoblast function, Peak Bone Mass achievement and Bioavailability. J Nutr Biochem. 22(4):318-327.PMID: 20579866 (Impact Factor-4.35) |
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51. Kunal Sharan, Gaurav Swarnkar, Jawed A Siddique, Avinash Kumar, Preeti Rawat, Manmeet Kumar, Geet K Nagar, Lakshmi Manickavasagam, Sheelendra P Singh; Geetanjali Mishra, Wahajuddin, Girish K Jain, Rakesh Maurya, Naibedya Chattopadhyay (2010) A Novel Flavonoid, 6-C-β-D-glucopyranosyl-(2S,3S)-(+)-3',4',5,7-tetrahydroxyflavanone, Isolated From Ulmus wallichiana-Planchon Mitigates Ovariectomy-induced Osteoporosis in Rats. Menopause. 17(3): 577-86. PMID: 20393370(Impact Factor-3.45) |
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52. Wahajuddin*, Sheelendra Pratap Singh* and Girish Kumar Jain (2010) Determination and Pharmacokinetic Study of Biochanin A and Its Metabolite Genistein in Rat Plasma by Liquid Chromatography–Mass Spectrometry. Medicinal Chemistry Research, 19:S136-S137. (*Authors contributed equally) (Impact Factor-1.1) |
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